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GHK-Cu chelates copper(II) with a dissociation constant of approximately 10^-16.44 M, but peptide degradation in culture media can release free copper ions that generate ROS through Fenton-type reactions

In a rat model of IC/BPS, magnesium L-threonate (a form of magnesium that crosses the bloodbrain barrier) reduced signs of pain hypersensitivity and depressive behaviors

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This observation aligns with previous work showing that pharmacological inhibition or genetic ablation of NMDA receptors in agouti-related-protein (AgRP)-expressing neurons alters synaptic adaptation to fasting 39,40